Haynes Lab:Notebook/Engineering PC-TFs/2014/09/15: Difference between revisions

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RFP signal
RFP signal
* Followed the fluorescent protein assay
* Followed the fluorescent protein assay
* Used 100 μL per well
* Results:
* Results:


Line 31: Line 32:


Bradford assay
Bradford assay
* Used 5 μL of each sample
* Used 5 μL for each BL09 sample
* OD 590 readings:
 
# Standard 1 (0 μg) = 0.25
# Standard 2 (1 μg) = 0.266
# Standard 3 (0 μg) = 0.295
# Standard 4 (0 μg) = 0.356
# Standard 5 (0 μg) = 0.432
# Standard 6 (0 μg) = 0.608
# BL09 1 = 0.486
# BL09 2 = 0.481
# BL09 negative = 0.471
 
Standard curve
 
[[Image:CG091514_Bradford.jpg|300px]]
 
 
Final results:
 
{|
|-
| Sample


# standard 1 (1 ug) = 0.25 0.266 0.295 0.356 0.432 0.608 0.486 0.481 0.471




Revision as of 14:09, 15 September 2014



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Summary

  • Protein extraction
  • Plate reader measurements: RFP and Bradford (protein)


Protein extraction from transfected cells

  • Cameron, please add notes here


Plate reader measurements

RFP signal

  • Followed the fluorescent protein assay
  • Used 100 μL per well
  • Results:
  1. BL09 1 = 109
  2. BL09 2 = 610
  3. BL09 negative ctrl (no DNA) = 47


Bradford assay

  • Used 5 μL for each BL09 sample
  • OD 590 readings:
  1. Standard 1 (0 μg) = 0.25
  2. Standard 2 (1 μg) = 0.266
  3. Standard 3 (0 μg) = 0.295
  4. Standard 4 (0 μg) = 0.356
  5. Standard 5 (0 μg) = 0.432
  6. Standard 6 (0 μg) = 0.608
  7. BL09 1 = 0.486
  8. BL09 2 = 0.481
  9. BL09 negative = 0.471

Standard curve


Final results:

Sample




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