Haynes Lab:Notebook/Engineering PC-TFs/2014/09/15

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Summary

  • Protein extraction
  • Plate reader measurements: RFP and Bradford (protein)


Protein extraction from transfected cells

  • Cameron, please add notes here


Plate reader measurements

RFP signal

  • Followed the fluorescent protein assay
  • Results:
  1. BL09 1 = 109
  2. BL09 2 = 610
  3. BL09 negative ctrl (no DNA) = 47


Bradford assay

  • Used 5 μL of each sample
  1. standard 1 (1 ug) = 0.25 0.266 0.295 0.356 0.432 0.608 0.486 0.481 0.471



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