Haynes Lab:Notebook/Engineering PC-TFs/2014/09/15: Difference between revisions

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Final results:
Final results:


{|
{| {{table}}
| Sample||Protein conc.||Protein*100 μL||Raw Fluor. (100 uL)||Fluor/ μg protein
|-
|-
| Sample
| BL09 1||10.42||1041.96||109||0.104610111
|-
| BL09 2||10.20||1019.64||610||0.598248687
|-
| BL09 negative||9.75||975.00||47||0.048205128
|}




Revision as of 14:30, 15 September 2014



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Summary

  • Protein extraction
  • Plate reader measurements: RFP and Bradford (protein)


Protein extraction from transfected cells

  • Cameron, please add notes here


Plate reader measurements

RFP signal

  • Followed the fluorescent protein assay
  • Used 100 μL per well
  • Results:
  1. BL09 1 = 109
  2. BL09 2 = 610
  3. BL09 negative ctrl (no DNA) = 47


Bradford assay

  • Used 5 μL for each BL09 sample
  • OD 590 readings:
  1. Standard 1 (0 μg) = 0.25
  2. Standard 2 (1 μg) = 0.266
  3. Standard 3 (0 μg) = 0.295
  4. Standard 4 (0 μg) = 0.356
  5. Standard 5 (0 μg) = 0.432
  6. Standard 6 (0 μg) = 0.608
  7. BL09 1 = 0.486
  8. BL09 2 = 0.481
  9. BL09 negative = 0.471

Standard curve


Final results:

Sample Protein conc. Protein*100 μL Raw Fluor. (100 uL) Fluor/ μg protein
BL09 1 10.42 1041.96 109 0.104610111
BL09 2 10.20 1019.64 610 0.598248687
BL09 negative 9.75 975.00 47 0.048205128




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