Haynes Lab:Notebook/Engineering PC-TFs/2014/10/11: Difference between revisions
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# Warmed selection agar plates at 37°C. | # Warmed selection agar plates at 37°C. | ||
# Incubated DH5α Turbo competent cells on ice just until thawed. Use 50 μL per transformation. | # Incubated DH5α Turbo competent cells on ice just until thawed. Use 50 μL per transformation. | ||
# Added 50 μL thawed cells to | # Added 50 μL thawed cells to 5μL LCR product. Immediately placed on ice and incubated for 10 min. (Do not heat shock; No 30 min. recovery is required for Amp resistance) | ||
# | # Pipetted the total volume of cells + LCR reaction onto the agar; spread using sterile glass beads. | ||
# | # Incubated overnight at 37°C. | ||
Revision as of 19:48, 11 October 2014
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Summary
Purified BL09 Negative Control Protein Extract
Digest
LCR
Transformation
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