Haynes Lab:Notebook/Engineering PC-TFs/2014/11/05: Difference between revisions
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'''Normal Transformation (BL01 & BL05 in V0120)''' | '''Normal Transformation (BL01 & BL05 in V0120)''' | ||
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* Warmed selection agar plates at 37°C. | |||
* Incubated DH5α Turbo competent cells on ice just until thawed. Use 50 μL per transformation. | |||
* Added 50 μL thawed cells to 5μL LCR product. Immediately placed on ice and incubated for 10 min. (Do not heat shock; No 30 min. recovery is required for Amp resistance) | |||
* Pipetted the total volume of cells + LCR reaction onto the agar; spread using sterile glass beads. | |||
* Incubated overnight at 37°C. | |||
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Revision as of 21:09, 5 November 2014
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Summary
Gel Extraction/Purification of BL01
Gel Purification: BL01/V0120
Concentration
LCR
Normal Transformation (BL01 & BL05 in V0120)
Long Transformation
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