Haynes Lab:Notebook/Engineering PC-TFs/2015/01/26: Difference between revisions

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<u>BL05 [XbaI/SpeI]</u><br>  
<u>BL05 [XbaI/SpeI]</u><br>  
Forward insertion: 3659, 4064 <br>
bl05 insert: 2630 <br>
Reverse insertion: 1538, 6185 <br>
v0120 vector: 1538, 6185 <br>
No insertion: 1538, 4064 <br> <br>
XbaI cut CMV/MV9: 5197 <br> <br>
<u>CMV/MV9 with [XbaI] </u> <br>
<u>CMV/MV9 with [XbaI] </u> <br>
5197<br><br>
5197<br><br>


| rowspan="10" | [[|400px|Today's gel]]<br>
| rowspan="10" | [[Image:1-26-2014 bl05 purification and cmvmv9.JPG|400px|Today's gel]]<br>
30μL/lane; 1% agarose;<br>  
30μL/lane; 1% agarose;<br>  
<u>Observed lengths:</u> <br>
<u>Observed lengths:</u> <br>

Revision as of 15:04, 26 January 2015



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Summary

Gel Extraction

Reagent Volume

Expected:
BL05 [XbaI/SpeI]
bl05 insert: 2630
v0120 vector: 1538, 6185
XbaI cut CMV/MV9: 5197

CMV/MV9 with [XbaI]
5197

 Today's gel

30μL/lane; 1% agarose;
Observed lengths:
W2 BL05 S1
W3 BL05 S2
W4 CMV/MV9 S1P(urple)
W5 CMV/MV9 S1B(lue)

Ladder

DNA[BL05: W2,W3*](CM9: W4,W5) [8.0 μL] (5.0 μL)
10X buffer 3.0 μL
XbaI 1.0 μL
SpeI 1.0 μL (0uL)
dH2O [17μL] (21μL)
Total 30 μL --> 37°C/ 30 min.




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