Haynes Lab:Notebook/Engineering PC-TFs/2015/01/26: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 46: Line 46:
|}
|}
<br>
<br>
 
<br>
'''BL05 Gel Purification and CMV/MV9 XbaI Cut Vector Concentrations'''<br>
{| {{table}} border="1" cellspacing="3" <!-- Plates. table -->
|- valign="top"
| rowspan="7" | [[Image:Bl05 gel purification concentration.png|400px|Hover name]]
|}
<br>




Revision as of 09:29, 28 January 2015



 		<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

Gel Extraction

Reagent Volume

Expected:
BL05 [XbaI/SpeI]
bl05 insert: 2630
v0120 vector: 1538, 6185
XbaI cut CMV/MV9: 5197

CMV/MV9 with [XbaI]
5197

 Today's gel

30μL/lane; 1% agarose;
Observed lengths:
W2 BL05 S1
W3 BL05 S2
W4 CMV/MV9 S1P(urple)
W5 CMV/MV9 S1B(lue)

Ladder

DNA[BL05: W2,W3*](CM9: W4,W5) [8.0 μL] (5.0 μL)
10X buffer 3.0 μL
XbaI 1.0 μL
SpeI 1.0 μL (0uL)
dH2O [17μL] (21μL)
Total 30 μL --> 37°C/ 30 min.



BL05 Gel Purification and CMV/MV9 XbaI Cut Vector Concentrations

Hover name




Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2015/01/26&oldid=855542"