Haynes Lab:Notebook/Engineering PC-TFs/2015/03/24: Difference between revisions
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==Summary== | ==Summary== | ||
* | * Liquid Cultures | ||
'''Picking Colonies; Liquid Cultures'''<br> | |||
No growth on any LCR plates (likely due to linearized vector dilution or old parts).<br> | |||
~100 colonies on successfully constructed PcTF (BL09, BL01) and BL05 in V0120 retransformation plates.<br> | |||
No growth on either negative (new amp seems to have fixed this).<br> | |||
Two colonies picked from each retransformation plate.<br> | |||
Grew up clones in 4mL LB amp media in shaker overnight. | |||
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Latest revision as of 00:52, 27 September 2017
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Summary
Picking Colonies; Liquid Cultures |