Haynes Lab:Notebook/Engineering PC-TFs/2015/05/05: Difference between revisions
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BBa_J176132 KAH024 240 PLrigid4, 4x peptide linker, rigid; (EAAAR)16; alpha helix secondary structure; see Yan et al. 2007 <br> | BBa_J176132 KAH024 240 PLrigid4, 4x peptide linker, rigid; (EAAAR)16; alpha helix secondary structure; see Yan et al. 2007 <br> | ||
BBa_S04707 KAH90 1053 fshPCD : [mCh : VP64] DNA <br><br> | BBa_S04707 KAH90 1053 fshPCD : [mCh : VP64] DNA <br><br> | ||
Used 1μL plasmid DNA (concentration ~200ng/μL), 10μL dH<sub>2</sub>O, and 50μL DH5α-Turbo cells. Incubated mixture for 5 minutes on ice and then plated on warmed labeled Amp plates. | Used 1μL plasmid DNA (concentration ~200ng/μL), 10μL dH<sub>2</sub>O, and 50μL DH5α-Turbo cells. Incubated mixture for 5 minutes on ice and then plated on warmed labeled Amp plates. Water and cells served as the negative control. | ||
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Revision as of 14:08, 6 May 2015
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