Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/03/26: Difference between revisions
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<b>Procedure</b> | <b>Procedure</b> | ||
Follow the instructions in the [http://openwetware.org/wiki/Image:Gblocks-user-guide.pdf gBlock user guide. | Follow the instructions in the [http://openwetware.org/wiki/Image:Gblocks-user-guide.pdf gBlock user guide], under 'Cohesive-end restriction cloning'. | ||
Revision as of 11:18, 26 March 2015
 Project name
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Entry titleToday I'm going to try restriction digest of the gBlock and the vector, followed by ligation and transformation into DH5α competent E. coli cells. Procedure Follow the instructions in the gBlock user guide, under 'Cohesive-end restriction cloning'.
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