Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/30
From OpenWetWare
Ligation/Amplification of DBN002 & DBN003 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OverviewGoing to try Type IIS Digestion/Ligation of DBN002 & DBN003, followed by PCR amplification. Type IIS Digestion/Ligation
Incubate the ligation reaction in a thermocycler:
Clean-up of IIS LigationUse the Qiagen kit. PCR
|