Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/05/20: Difference between revisions
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(Autocreate 2015/05/20 Entry for Haynes_Lab:Notebook/HPK-CFP_insertion_into_Gal4EED/Luc_using_CRISPR) |
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Today's project is...</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Today's project is...</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Overview== | ==Overview== | ||
Ordered new primers for PCR amplification of the DBN006 fragment. They're now matched to have similar melting temperatures and have a larger anchor on either side of the BsaI binding site. Primer names are DBN006 f1 and DBN006 r1. | |||
Latest revision as of 00:58, 27 September 2017
Today's project is... | Main project page Previous entry Next entry |
OverviewOrdered new primers for PCR amplification of the DBN006 fragment. They're now matched to have similar melting temperatures and have a larger anchor on either side of the BsaI binding site. Primer names are DBN006 f1 and DBN006 r1.
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