Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2016/01/12
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OverviewTaking the following steps today:
EM7:ZeoR PCRPrimers:
Reaction setup:
Prepare master mix:
PCR extension times:
DNA QuantificationConcentration is measured at 245 ng/µL with 260/280 around 1.9. |