Haynes Lab:Notebook/Heathers Lab Notebook/2013/07/24: Difference between revisions
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(Autocreate 2013/07/24 Entry for Haynes_Lab:Notebook/Heathers_Lab_Notebook) |
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== | =='''Second Plasmid Procedure'''== | ||
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* Plasmid KA14 was selected. | |||
*Added 9μL of sterile H2O, following with 1μL plasmid (Note: The plasmid was almost empty, so the volume may have been less) to tube | |||
*Competent DH5-Alpha cells were obtained and thawed in an ice bath for ten minutes. | |||
*50μL of these cells were then pipetted to the tube with plasmid and water for a total volume of around 60-70μL. The remaining cells were disposed. | |||
*Prewarmed agar plates were labeled with Amp resistance, the date 07/24/2013, initials HB, the strain and KA14. The total volume of cells were pipetted into agar plates using a measurement of 70μL. | |||
*Six sterile glass beads were used to spread the cells. The plates were then placed in the incubator at 37 degrees C for 12 hours. | |||
Revision as of 23:58, 25 July 2013
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Second Plasmid Procedure
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