Haynes Lab:Notebook/Inteins/2013/02/19: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Amanda Ispas (talk | contribs) (Autocreate 2013/02/19 Entry for Haynes_Lab:Notebook/Inteins) |
Amanda Ispas (talk | contribs) |
||
Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Entry title== | ==Entry title== | ||
* | * Completed a restriction/digest | ||
*Incubated @ 37.5- 37 degrees C for 20 mins. | |||
*Completed a gel | |||
*1KBT 9(186) 9(160) 10(116) 10(106) | |||
*8.5 of the ladder, 30 uL for dna wells | |||
*Also did a gel extraction | |||
**300 uL ADB Buffer of every gel slice | |||
**Incubate @ 50 degrees C for 3 min., vortex, put back for another 3 min., repeat until melted | |||
**Follow the gel dna recovery kit | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 14:34, 5 March 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Entry title
|