The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
|
Entry title
- Completed a restriction/digest from really good mini prep!
- Used brand new enzymes for the restriction/ digest
- Made a brand new gel
1KBT KAH9(186) KAH9(160) KAH10 (116) KAH10 (106)
- Put 10 of ladder, 30 in other wells.
http://openwetware.org/wiki/Image:3-1-2013_gel.jpg
- Run dna recovery kit
- Melted @ 55.6 degrees C for 6 min., vortex in between
- 8uL + 7 water to elute dna in last step
- Completed ligation/transformation
- Used both 2x and 10x buffers
- Get competent cells and keep on ice
- Used both buffers to see if lab shared stock Roche ligation buffer was contaminated.
- Used brand new ligase and ligation buffer
- Left ligations for at least 30 mins.
- Add 50 uL of competent cells
|