Haynes Lab:Notebook/Inteins/2013/03/08: Difference between revisions

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==Entry title==
==Entry title==
* Insert content here...
René:<br>
 
Added some cmp to some old cmp plates. Added 25ul liquid cmp stock to 225ul of water, added to plates, spread with beads, left on bench for about 2 hours then put at 4deg<br>
transfered the colonies on amp intein plates to new amp plates using parafilm. kept the old ones just in case. if nothing grows, I'll try retransforming<br>
picked colonies from B0034+C0061 (RBS+LuxI) and B0034+C0078 (RBS+LasI) plates (transformed yesterday from Fuqing). Growing in 4ml LB+amp.<br><br>
KAH9 and KAH10 assembly:<br>
Colony 2, the one that looked like it worked on the colony PCR, hasn't grown much after leaving the plate overnight in the incubator so it's out on my bench.


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Latest revision as of 22:32, 26 September 2017

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Entry title

René:
Added some cmp to some old cmp plates. Added 25ul liquid cmp stock to 225ul of water, added to plates, spread with beads, left on bench for about 2 hours then put at 4deg
transfered the colonies on amp intein plates to new amp plates using parafilm. kept the old ones just in case. if nothing grows, I'll try retransforming
picked colonies from B0034+C0061 (RBS+LuxI) and B0034+C0078 (RBS+LasI) plates (transformed yesterday from Fuqing). Growing in 4ml LB+amp.

KAH9 and KAH10 assembly:
Colony 2, the one that looked like it worked on the colony PCR, hasn't grown much after leaving the plate overnight in the incubator so it's out on my bench.


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