Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/02: Difference between revisions

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Measure concentrations
Measure concentrations


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Revision as of 07:33, 2 November 2012

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November 2, 2012 Digestion and PCR

  • Check LOV and H2B-GFP minipreps with digestion
  • BioBrick PCR

Minipreps

Measure concentrations

Sample 260/280 ng/μL
LOV 1.834 281.868
H2B-GFP 1.87 118.217

Check miniprep results

  • Use a plasmid editor software to check sequences for restriction digest enzymes
  • LOV has two PstI sites while H2B-GFP has one PstI, one NotI, and two EcoRI sites
  • Decide to cut LOV with PstI and H2B-GFP with PstI and EcoRI
  • Expected bands for LOV at 251 with PstI digest
  • Expected bands for H2B-GFP at 4885, 205 with Psti and EcoRI digest


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