Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/02: Difference between revisions
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| <u>Reagent</u> || <u>LOV</u> || <u>H2B-GFP/ PstI</u> || <u>H2B-GFP/ EcoRI</u> | | <u>Reagent</u> || <u>LOV</u> || <u>H2B-GFP/ PstI</u> || <u>H2B-GFP/ EcoRI</u> | ||
|- | |- | ||
| DNA(plasmid) || 2.0 μL || 2.0 || 2.0 | | DNA(plasmid) || 2.0 μL || 2.0 || 2.0 | ||
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| || 15.0 || 15.0 || 15.0 | | || 15.0 || 15.0 || 15.0 | ||
|} | |||
'''Results''' | |||
{| class="wikitable" border=0 | |||
|- | |||
| <u>Expected:</u><br>1. LOV = 251, 3639<br>2. H2B-GFP/ PstI = 4885, 205<br>3. H2B-GFP/ EcoRI = ? | |||
| [[Image:Vi110212_gel1.tif|150px|Digest check 11/02/12]]<br>15 μL/lane; 1% agarose | |||
|} | |} | ||
Revision as of 08:49, 2 November 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||
November 2, 2012 Digestion and PCR
Minipreps Measure concentrations
Check miniprep results
Results
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