Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/06

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(Autocreate 2012/11/06 Entry for Haynes_Lab:Notebook/Investigating_Photo-Switchable_Synthetic_Nucleosomes)
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==Entry title==
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==November 6, 2012==
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* Insert content here...
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* Restriction digests of H2B-GFP and LOV
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{|border="1" cellpadding="5" cellspacing="0" align="center"
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|-
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! scope="col" style="background:#efefef;" | Reagent
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! scope="col" style="background:#efefef;" | H2B-GFP/EcoRI
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! scope="col" style="background:#efefef;" | H2B-GFP/PstI
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! scope="col" style="background:#efefef;" | LOV/EcoRI
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|-
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|DNA(plasmid)
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|2.0
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|2.0
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|2.0
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|-
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|10x buffer
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|1.5
 +
|1.5
 +
|1.5
 +
|-
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|EcoRI
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|1
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|---
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|1
 +
|-
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|PstI
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|---
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|1
 +
|---
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|dH<sub>2</sub>O
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|10.5
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|10.5
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|10.5
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|-
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|Total
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|15
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|15
 +
|15
 +
|}
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 +
[[Image:Vi110212_gel1.tif|150px|Digest check 11/02/12]
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Revision as of 10:38, 7 November 2012

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November 6, 2012

  • Restriction digests of H2B-GFP and LOV
Reagent H2B-GFP/EcoRI H2B-GFP/PstI LOV/EcoRI
DNA(plasmid) 2.0 2.0 2.0
10x buffer 1.5 1.5 1.5
EcoRI 1
1
PstI
1
dH2O 10.5 10.5 10.5
Total 15 15 15

[[Image:Vi110212_gel1.tif|150px|Digest check 11/02/12]


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