Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/06

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(November 6, 2012)
(November 6, 2012)
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  |-
  |-
  |EcoRI
  |EcoRI
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  |1
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  |1.0
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  |1.0
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  |PstI
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  |15
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  |15.0
  |}
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Revision as of 10:40, 7 November 2012

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November 6, 2012

  • Restriction digests of H2B-GFP and LOV
Reagent H2B-GFP/EcoRI H2B-GFP/PstI LOV/EcoRI
DNA(plasmid) 2.0 2.0 2.0
10x buffer 1.5 1.5 1.5
EcoRI 1.0 0 1.0
PstI 0 1.0 0
dH2O 10.5 10.5 10.5
Total 15.0 15.0 15.0

[[Image:Vi110212_gel1.tif|150px|Digest check 11/02/12]


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