Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/06

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  ! scope="col" style="background:#efefef;" | H2B-GFP/EcoRI
  ! scope="col" style="background:#efefef;" | H2B-GFP/EcoRI
  ! scope="col" style="background:#efefef;" | H2B-GFP/PstI
  ! scope="col" style="background:#efefef;" | H2B-GFP/PstI
-
  ! scope="col" style="background:#efefef;" | LOV/EcoRI
+
  ! scope="col" style="background:#efefef;" | LOV/PstI
  |-
  |-
  |DNA(plasmid)   
  |DNA(plasmid)   
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  |1.0
  |1.0
  |0
  |0
-
  |1.0
+
  |0
  |-
  |-
  |PstI
  |PstI
  |0
  |0
  |1.0
  |1.0
-
  |0
+
  |1.0
  |-
  |-
  |dH<sub>2</sub>O
  |dH<sub>2</sub>O

Revision as of 10:56, 7 November 2012

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November 6, 2012

  • Restriction digests of H2B-GFP and LOV
Restriction Digest Reagents
Reagent H2B-GFP/EcoRI H2B-GFP/PstI LOV/PstI
DNA(plasmid) 2.0 2.0 2.0
10x buffer 1.5 1.5 1.5
EcoRI 1.0 0 0
PstI 0 1.0 1.0
dH2O 10.5 10.5 10.5
Total 15.0 15.0 15.0


Expected:
1. H2B-GFP/ EcoRI = 4885, 210
2. H2B-GFP/ PstI = 5101
3. LOV/PstI = 251


Results of the digests are shown.
Results of the digests are shown.


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