Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/06

(Difference between revisions)

Revision as of 16:26, 7 November 2012

Project name

Restriction Digest Reagents
Reagent	H2B-GFP/EcoRI	H2B-GFP/PstI	LOV/PstI
DNA(plasmid)	2.0	2.0	2.0
10x buffer	1.5	1.5	1.5
EcoRI	1.0	0	0
PstI	0	1.0	1.0
dH₂O	10.5	10.5	10.5
Total	15.0	15.0	15.0

Expected: 1. H2B-GFP/ EcoRI = 4885, 210 2. H2B-GFP/ PstI = 5101 3. LOV/PstI = 251

Results of the digests are shown.

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-	[[Image:~~V.N_Gel_11~~-6-12.png\|thumb\|frame\|center\|Results of the digests are shown.]]	+	[[Image:VN_Gel_11-6-12.png\|thumb\|frame\|center\|Results of the digests are shown.]]

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