Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/06

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(→November 6, 2012)
(→November 6, 2012)
Line 56: Line 56:
<center>
<center>
-
[[Image:VN_Gel_11-6-12.png|100px|Results of the digests are shown.]]
+
[[Image:VN_Gel_11-6-12.png|150px|Results of the digests are shown.]]
[[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]]
[[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]]
</center>
</center>

Revision as of 16:43, 7 November 2012

Project name Main project page
Previous entry      Next entry

November 6, 2012

  • Restriction digests of H2B-GFP and LOV
Restriction Digest Reagents
Reagent H2B-GFP/EcoRI H2B-GFP/PstI LOV/PstI
DNA(plasmid) 2.0 2.0 2.0
10x buffer 1.5 1.5 1.5
EcoRI 1.0 0 0
PstI 0 1.0 1.0
dH2O 10.5 10.5 10.5
Total 15.0 15.0 15.0


Expected:
1. H2B-GFP/ EcoRI = 4885, 210
2. H2B-GFP/ PstI = 5101
3. LOV/PstI = 251

Results of the digests are shown. Image:KAH_Fermentas_GeneRuler_1kbplus.jpg


Retrieved from "http://www.openwetware.org/wiki/Haynes_Lab:Notebook/Investigating_Photo-Switchable_Synthetic_Nucleosomes/2012/11/06"
Personal tools