Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/06: Difference between revisions

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| <u>Expected:</u><br>1. LOV/PstI = 251<br>2. H2B-GFP/ PstI = 5101<br>3. H2B-GFP/ EcoRI = 4885, 210
| <u>Expected:</u><br>1. H2B-GFP/ EcoRI = 4885, 210 <br>2. H2B-GFP/ PstI = 5101<br>3. LOV/PstI = 251
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Revision as of 07:55, 7 November 2012

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November 6, 2012

  • Restriction digests of H2B-GFP and LOV
Restriction Digest Reagents
Reagent H2B-GFP/EcoRI H2B-GFP/PstI LOV/EcoRI
DNA(plasmid) 2.0 2.0 2.0
10x buffer 1.5 1.5 1.5
EcoRI 1.0 0 1.0
PstI 0 1.0 0
dH2O 10.5 10.5 10.5
Total 15.0 15.0 15.0


Expected:
1. H2B-GFP/ EcoRI = 4885, 210
2. H2B-GFP/ PstI = 5101
3. LOV/PstI = 251


Results of the digests are shown.


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