Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/06
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Investigating Photo-Switchable Synthetic Nucleosomes
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November 6, 2012
Restriction digests of H2B-GFP and LOV
Restriction Digest Reagents
Reagent
H2B-GFP/EcoRI
H2B-GFP/PstI
LOV/EcoRI
DNA(plasmid)
2.0
2.0
2.0
10x buffer
1.5
1.5
1.5
EcoRI
1.0
0
1.0
PstI
0
1.0
0
dH
2
O
10.5
10.5
10.5
Total
15.0
15.0
15.0
Results of the digests are shown.
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Contributing Authors
Vi Nguyen
Karmella Haynes