Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/13: Difference between revisions
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* Run entire 30 μL on a 1% gel (use the big fat-tooth well comb) | * Run entire 30 μL on a 1% gel (use the big fat-tooth well comb) | ||
* Cut band out of gel (use UV box); H2B = ~410, LOV = ~461, pSB1A2 = ~2000 | * Cut band out of gel (use UV box); H2B = ~410, LOV = ~461, pSB1A2 = ~2000 | ||
<center> | |||
[[Image:VN_Gel_11-14-12.png|200px|Results of from the digests are shown.]] | |||
[[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]] | |||
</center> | |||
<center> | |||
Results of the enzyme digests are shown, with H2B on the left, LOV in the middle, and pSB1A3 on the right. The gel was loaded with 30 μL samples. The bands that were cut are outlined in green. | |||
</center> | |||
* Extract DNA from gel | * Extract DNA from gel | ||
Revision as of 05:00, 14 November 2012
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November 13, 2012
Results of the enzyme digests are shown, with H2B on the left, LOV in the middle, and pSB1A3 on the right. The gel was loaded with 30 μL samples. The bands that were cut are outlined in green.
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