Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/13

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2012/11/13 Entry for Haynes_Lab:Notebook/Investigating_Photo-Switchable_Synthetic_Nucleosomes)
(Entry title)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==November 13, 2012==
-
* Insert content here...
+
* Digests:
 +
** In a 30 μL reaction, cut H2B with XbaI and SpeI
 +
** In a 30 μL reaction, cut LOV with XbaI and SpeI
 +
** In a 30 μL reaction, cut a [http://partsregistry.org/Part:pSB1A2 pSB1A2 plasmid] (from Rene) XbaI and SpeI
 +
 
 +
{| class="wikitable" border=1 cellpadding="5" cellspacing="0"
 +
|-
 +
| Reagent || H2B|| LOV || pSB1A2
 +
|-
 +
| DNA || 8.0 || 8.0 || 15.0
 +
|-
 +
| XbaI || 1.0|| 1.0 || 1.0
 +
|-
 +
| SpeI || 1.0 || 1.0 || 1.0
 +
|-
 +
| 10x buffer || 3.0 || 3.0 || 3.0
 +
|-
 +
| dH<sub>2</sub>O || 10.0 || 10.0 || 10.0
 +
|-
 +
| Total vol. || 23. 0 || 23.0  || 30.0
 +
|}
 +
 
 +
 
 +
* Run entire 30 μL on a 1% gel (use the big fat-tooth well comb)
 +
* Cut band out of gel (use UV box); H2B = ~410, LOV = ~461, pSB1A2 = ~2000
 +
* Extract DNA from gel
 +
 

Revision as of 00:19, 14 November 2012

Project name Main project page
Previous entry      Next entry

November 13, 2012

  • Digests:
    • In a 30 μL reaction, cut H2B with XbaI and SpeI
    • In a 30 μL reaction, cut LOV with XbaI and SpeI
    • In a 30 μL reaction, cut a pSB1A2 plasmid (from Rene) XbaI and SpeI
Reagent H2B LOV pSB1A2
DNA 8.0 8.0 15.0
XbaI 1.0 1.0 1.0
SpeI 1.0 1.0 1.0
10x buffer 3.0 3.0 3.0
dH2O 10.0 10.0 10.0
Total vol. 23. 0 23.0 30.0


  • Run entire 30 μL on a 1% gel (use the big fat-tooth well comb)
  • Cut band out of gel (use UV box); H2B = ~410, LOV = ~461, pSB1A2 = ~2000
  • Extract DNA from gel



Personal tools