Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/14: Difference between revisions
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| Vector DNA (50 ng) || 5.0 || 5.0 || 5.0 | | Vector DNA (50 ng) || 5.0 || 5.0 || 5.0 | ||
|- | |- | ||
| 2x Roche Rapid Ligation buffer || 7.0 | | 2x Roche Rapid Ligation buffer || 7.0 || 7.0 || 7.0 | ||
|- | |- | ||
| New England Biolabs T4 ligase || 1.0 || 1.0 || 1.0 | | New England Biolabs T4 ligase || 1.0 || 1.0 || 1.0 |
Revision as of 19:46, 15 November 2012
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November 14, 2012
Refer to this page for a full description: http://openwetware.org/wiki/Haynes:Assembly101
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 |