Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/21: Difference between revisions
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* Cut band out of gel (use UV box) | * Cut band out of gel (use UV box) | ||
[[Image:VN_Gel_11-14-12.png|200px|Results of from the digests are shown.]] | [[Image:VN_Gel_11-14-12.png|200px|Results of from the digests are shown.]] | ||
[[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]] | [[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]] | ||
# H2B = 410 | # H2B = 410 | ||
# LOV = 461 | # LOV = 461 | ||
'''Dephosphorylation''' | '''Dephosphorylation''' |
Revision as of 14:05, 21 November 2012
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11/21/12
PCR program:
Results of the PCR products, with H2B on the left and LOV on the right. The gel was loaded with 5 μL samples.
Assemblies
Ligation
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101
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