Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/21: Difference between revisions
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* 4°C ∞ | * 4°C ∞ | ||
* Clean up PCR products using the Zymo Clean and Concentrator kit | |||
* Be sure to elute using 15 μL dH<sub>2</sub>O | |||
</ | |||
---- | ---- | ||
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| Reagent || H2B PCR || LOV PCR | | Reagent || H2B PCR || LOV PCR | ||
|- | |- | ||
| DNA || | | DNA || 15.0 || 15.0 | ||
|- | |- | ||
| XbaI || 1.0|| 1.0 | | XbaI || 1.0|| 1.0 |
Revision as of 14:20, 21 November 2012
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11/21/12
PCR program:
Assemblies
Dephosphorylation > Dephosphorylation (Roche)
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101
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