Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/21: Difference between revisions
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'''Dephosphorylation''' | '''Dephosphorylation''' | ||
> Dephosphorylation (Roche) | |||
# pSB1A3 - X/S = | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Dephos table --> | |||
|- | |||
| <u>Reagent</u> || <u>Volume</u> | |||
|- | |||
| DNA (clean digest) || up to 17 μL (500 ng) | |||
|- | |||
| 10x buffer d.p. || 2.0 | |||
|- | |||
| phosphatase || 1.0 | |||
|- | |||
| dH<sub>2</sub>O || --- | |||
|- | |||
| || 20 μL --> 37°C/ 10 min.; 75°C/ 2 min.; [final] = 25 ng/μL | |||
|} | |||
Revision as of 14:07, 21 November 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11/21/12
PCR program:
Results of the PCR products, with H2B on the left and LOV on the right. The gel was loaded with 5 μL samples.
Assemblies
> Dephosphorylation (Roche)
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101
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