Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/21: Difference between revisions
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* Incubate at 37°C/ 10 min. | * Incubate at 37°C/ 10 min. | ||
* Heat inactivate at 75°C/ 2 min. | * Heat inactivate at 75°C/ 2 min. | ||
* [final] = 200 ng/ | * [final] = 200 ng/μL / 15 μL = ~13 ng/μL | ||
Revision as of 14:18, 21 November 2012
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11/21/12
PCR program:
Results of the PCR products, with H2B on the left and LOV on the right. The gel was loaded with 5 μL samples.
Assemblies
Dephosphorylation > Dephosphorylation (Roche)
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101
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