Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/21: Difference between revisions
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==11/21/12== | ==11/21/12== | ||
'''Results from yesterday's ligation''' | |||
* Got 1:1 ratio of ligation: negative control for both | |||
* About 10 colonies total on every plate | |||
* Forgot to take into account X/S vector self-ligation | |||
* Will pick 4 colonies from each (H2B, LOV) to see if anything worked. | |||
* In the mean time, will retry cloning, trouble shoot procedure with help from Dr. Haynes | |||
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* Repeat PCR of H2B and LOV inserts | * Repeat PCR of H2B and LOV inserts |
Revision as of 14:26, 21 November 2012
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11/21/12Results from yesterday's ligation
PCR program:
Assemblies
Dephosphorylation > Dephosphorylation (Roche)
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101
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