Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/12/05

Current revision

Project name

Results from last 11/23/12 ligation

Performed table reactions twice: once for those used in H2B-LOV construct, once for those used in LOV-H2B construct

PCR program:

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 ==December 5, 2012==
-'''Results from last week's ligation'''
+'''Results from last 11/23/12 ligation'''
 * None of the colonies on any plates grew
 * Will retry cloning for each (H2B, LOV) by using the Gibson assembly method
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 * Be sure to elute using 15 μL dH<sub>2</sub>O
+{| class="wikitable" border=1 cellpadding="5" cellspacing="0"
+|-
+|Construct || Product || 260 || 280 || 260/280 || ng/μL
+|-
+|H2B-LOV || H2B 1 || 0.061 || 0.033 || 1.863 || 60.545
+|-
+|H2B-LOV || H2B 2 || 0.056 || 0.03 || 1.857 || 56.22
+|-
+|H2B-LOV || LOV 1 || 0.063 || 0.033 || 1.903 || 62.673
+|-
+|H2B-LOV || LOV 2 || 0.06 || 0.04 || 1.85 || 59.844
+|-
+|LOV-H2B || H2B 1 || 0.072 || 0.033 || 1.807 || 72.381
+|-
+|LOV-H2B || H2B 2 || 0.06 || 0.036 || 1.799 || 59.925
+|-
+|LOV-H2B || LOV 1 || 0.006 || 0.036 || 1.849 || 65.881
+|-
+|LOV-H2B || LOV 2 || 0.065 || 0.035 || 1.839 || 65.285
+|}
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