Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/12/08: Difference between revisions

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==Entry title==
==December 8, 2012==
* Insert content here...
'''Troubleshooting'''
* Check PCR products on a gel
** 1 μL PCR, 1 μL loading dye, 8 μL water
* Digest pSB1A3 again (30 mins)
** Gel isolation (larger band)
** Make sure gel is completely obliterated by vortexing and heating several times
* Transformation
** Use 50 ng backbone for Gibson with diluted PCR
** Use 10 μL for transformation




Revision as of 06:20, 7 May 2013

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December 8, 2012

Troubleshooting

  • Check PCR products on a gel
    • 1 μL PCR, 1 μL loading dye, 8 μL water
  • Digest pSB1A3 again (30 mins)
    • Gel isolation (larger band)
    • Make sure gel is completely obliterated by vortexing and heating several times
  • Transformation
    • Use 50 ng backbone for Gibson with diluted PCR
    • Use 10 μL for transformation



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