Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/12/08: Difference between revisions
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(Autocreate 2012/12/08 Entry for Haynes_Lab:Notebook/Investigating_Photo-Switchable_Synthetic_Nucleosomes) |
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== | ==December 8, 2012== | ||
* | '''Troubleshooting''' | ||
* Check PCR products on a gel | |||
** 1 μL PCR, 1 μL loading dye, 8 μL water | |||
* Digest pSB1A3 again (30 mins) | |||
** Gel isolation (larger band) | |||
** Make sure gel is completely obliterated by vortexing and heating several times | |||
* Transformation | |||
** Use 50 ng backbone for Gibson with diluted PCR | |||
** Use 10 μL for transformation | |||
Revision as of 06:20, 7 May 2013
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December 8, 2012Troubleshooting
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