Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/12/08

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(Autocreate 2012/12/08 Entry for Haynes_Lab:Notebook/Investigating_Photo-Switchable_Synthetic_Nucleosomes)
Current revision (09:20, 7 May 2013) (view source)
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==Entry title==
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==December 8, 2012==
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* Insert content here...
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'''Troubleshooting'''
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* Check PCR products on a gel
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** 1 μL PCR, 1 μL loading dye, 8 μL water
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* Digest pSB1A3 again (30 mins)
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** Gel isolation (larger band)
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** Make sure gel is completely obliterated by vortexing and heating several times
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* Transformation
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** Use 50 ng backbone for Gibson with diluted PCR
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** Use 10 μL for transformation

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December 8, 2012

Troubleshooting

  • Check PCR products on a gel
    • 1 μL PCR, 1 μL loading dye, 8 μL water
  • Digest pSB1A3 again (30 mins)
    • Gel isolation (larger band)
    • Make sure gel is completely obliterated by vortexing and heating several times
  • Transformation
    • Use 50 ng backbone for Gibson with diluted PCR
    • Use 10 μL for transformation



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