Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2013/02/08: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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* Final assembly product: pSB1A3 left end / H2B / LOV +His tag/ pSB1A3 right end | * Final assembly product: pSB1A3 left end / H2B / LOV +His tag/ pSB1A3 right end | ||
* Primer design | * Primer design | ||
H2B | #H2B | ||
1A3/H2B fwd 5'-cacaccaCGTCTCa TAGA ATGCCAGAGCCAGCG | 1A3/H2B fwd 5'-cacaccaCGTCTCa TAGA ATGCCAGAGCCAGCG | ||
H2B/LOV rev 5'-cacaccaCGTCTCa CCAA CTTAGCGCTGGTGTA | H2B/LOV rev 5'-cacaccaCGTCTCa CCAA CTTAGCGCTGGTGTA | ||
LOV | #LOV | ||
LOV fwd 5'-cacaccaCGTCTCa TTGGCTACTACACTT | LOV fwd 5'-cacaccaCGTCTCa TTGGCTACTACACTT | ||
LOV+his/1A3 rev 5'-cacaccaCGTCTCa TAGT ttagtggtgatggtgatgatgAAGTTCTTTTGCCGC | LOV+his/1A3 rev 5'-cacaccaCGTCTCa TAGT ttagtggtgatggtgatgatgAAGTTCTTTTGCCGC | ||
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<center> | <center> | ||
[[Image: | [[Image:VN_Gel_2-8-13.png|Results of the digests are shown.]] | ||
[[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]] | [[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]] | ||
</center> | </center> | ||
<center> | <center> | ||
Results of the PCR product digests are shown | Results of the PCR product digests are shown. It was later found that the transparency used was blocking the UV light, so no bands could be seen. | ||
</center> | </center> | ||
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| PCR Product || 260|| 280 || 260/280 || ng/μL | | PCR Product || 260|| 280 || 260/280 || ng/μL | ||
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| H2B || | | H2B || 0.078|| 0.043|| 1.81 || 77.995 | ||
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| LOV || 0.08 || 0.044 || 1.817 || 80.443 | |||
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| pSB1A3 || 0.023 || 0.014 || 1.727 || 23.386 | |||
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|} | |} | ||
Latest revision as of 22:25, 26 September 2017
Project name | Main project page Previous entry Next entry | |||||||||||||||||||||||||||||||||||||||||||
February 8, 2013Type IIS Assembly - Making PCR Products
1A3/H2B fwd 5'-cacaccaCGTCTCa TAGA ATGCCAGAGCCAGCG H2B/LOV rev 5'-cacaccaCGTCTCa CCAA CTTAGCGCTGGTGTA
LOV fwd 5'-cacaccaCGTCTCa TTGGCTACTACACTT LOV+his/1A3 rev 5'-cacaccaCGTCTCa TAGT ttagtggtgatggtgatgatgAAGTTCTTTTGCCGC
PCR program:
Results of the PCR product digests are shown. It was later found that the transparency used was blocking the UV light, so no bands could be seen.
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