Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2013/02/11: Difference between revisions
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(Autocreate 2013/02/11 Entry for Haynes_Lab:Notebook/Investigating_Photo-Switchable_Synthetic_Nucleosomes) |
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== | ==February 9, 2013== | ||
* | '''Golden Gate Assembly''' | ||
* Mediated assembly | |||
** The volume of purified DNA (x) needed to dilute in a final volume of 20 μL = length in bp ÷ measured ng/μL * 20 fmols/μL * 650 fg/fmol ÷ 1,000,000 fg/ng * 20 μL final volume | |||
** Formula: x = length in bp ÷ measured ng/μL * 0.013 * 20 | |||
{| class="wikitable" border=1 cellpadding="5" cellspacing="0" | |||
|- | |||
| DNA || length (bp) || ng/μL || vol. || dH<sub>2</sub>O | |||
|- | |||
| H2B || 410 || 77.995 || 1.4 || 18.6 | |||
|- | |||
| LOV || 461 || 80.443 || 1.5 || 18.5 | |||
|- | |||
| pSB1A3 || 2000 || 77.4 || 6.7 || 13.3 | |||
|} | |||
Revision as of 06:36, 7 May 2013
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February 9, 2013Golden Gate Assembly
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