Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2013/03/13: Difference between revisions
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| Reagent || Conc. || Volume | | Reagent || Conc. || Volume | ||
|- | |- | ||
| Tris-HCl (pH 7.8) || 300 mM || | | 1M Tris-HCl (pH 7.8) || 300 mM || 300.0 μL | ||
|- | |- | ||
| MgCl<sub>2</sub> || 100 mM || | | 1M MgCl<sub>2</sub> || 100 mM || 100.0 | ||
|- | |- | ||
| DTT || 100 mM || | | 1M DTT || 100 mM || 100.0 | ||
|- | |- | ||
| ATP || 10 mM || | | ATP || 10 mM || 10.0 | ||
|- | |- | ||
| Final vol. || || 1000 uL | | Final vol. || || 1000 uL |
Revision as of 21:11, 12 March 2013
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03/13/13
Golden Gate Assembly
1, 2. Promega - hPCD+BL01+pSB1A3
Note: *DNA is 20 fmole/μL Thermal cycler
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