Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2013/03/13: Difference between revisions
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{| {{table}} border="1" cellspacing="3" | {| {{table}} border="1" cellspacing="3" | ||
|- valign="top" bgcolor=#cfcfcf | |- valign="top" bgcolor=#cfcfcf | ||
| | | Reagent || Conc. || Volume | ||
|- | |- | ||
| | | Tris-HCl (pH 7.8) || 300 mM || ### | ||
|- | |- | ||
| | | MgCl<sub>2</sub> || 100 mM || ### | ||
|- | |- | ||
| | | DTT || 100 mM || ### | ||
|- | |||
| ATP || 10 mM || ### | |||
|- | |||
| Final vol. || || 1000 uL | |||
|} | |} | ||
Revision as of 21:09, 12 March 2013
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03/13/13
Golden Gate Assembly
1, 2. Promega - hPCD+BL01+pSB1A3
Note: *DNA is 20 fmole/μL Thermal cycler
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