Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2013/04/22: Difference between revisions
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'''Quikchange Site Directed Mutagenesis''' | '''Quikchange Site Directed Mutagenesis''' | ||
* The QuikChange Site-Directed Mutagenesis Kit (Catalog #200519) contains enough reagents for 10 total reactions, | * The QuikChange Site-Directed Mutagenesis Kit (Catalog #200519) contains enough reagents for 10 total reactions, which includes 5 control reactions. | ||
which includes 5 control reactions. | |||
* Thaw the dNTP mix once, prepare single-use aliquots, and store the aliquots at –20°C. Do not subject the dNTP mix | * Thaw the dNTP mix once, prepare single-use aliquots, and store the aliquots at –20°C. Do not subject the dNTP mix | ||
to multiple freeze-thaw cycles. | to multiple freeze-thaw cycles. |
Revision as of 06:55, 22 April 2013
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April 19, 2013Quikchange Site Directed Mutagenesis
to multiple freeze-thaw cycles.
|