Haynes Lab:Notebook/Jan/2013/08/12: Difference between revisions
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* 2. The reaction was set up as follows: | * 2. The reaction was set up as follows: | ||
Plasmid DNA 25.0 μl | Plasmid DNA 25.0 μl | ||
Fermentas FastDigest enzyme 1 1.0 μl | Fermentas FastDigest enzyme 1 1.0 μl | ||
Fermentas FastDigest enzyme 2 1.0 μl | Fermentas FastDigest enzyme 2 1.0 μl | ||
10x FastDigest buffer + green loading dye 3.0 μl | 10x FastDigest buffer + green loading dye 3.0 μl | ||
dH2O 0.0 μl | dH2O 0.0 μl | ||
30.0 μl total | 30.0 μl total | ||
The maximum amount of plasmid DNA was used and no dH2O was used because of the low concentration of the plasmid DNA (22 ng/μl). | The maximum amount of plasmid DNA was used and no dH2O was used because of the low concentration of the plasmid DNA (22 ng/μl). | ||
Revision as of 14:03, 21 August 2013
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Restriction Digest and Gel Electrophoresis
Plasmid DNA 25.0 μl Fermentas FastDigest enzyme 1 1.0 μl Fermentas FastDigest enzyme 2 1.0 μl 10x FastDigest buffer + green loading dye 3.0 μl dH2O 0.0 μl 30.0 μl total The maximum amount of plasmid DNA was used and no dH2O was used because of the low concentration of the plasmid DNA (22 ng/μl).
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