Haynes Lab:Notebook/Jan/2013/09/25

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(Plasmid Transformation)
 
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==Plasmid Transformation==
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==Plasmid Transformation of fshPCD==
* Plasmid name: fshPCD in V0120 vector
* Plasmid name: fshPCD in V0120 vector
** BioBrick name: BBa_J176002 [http://parts.igem.org/Part:BBa_J176002 BBa_J176002]
** BioBrick name: BBa_J176002 [http://parts.igem.org/Part:BBa_J176002 BBa_J176002]

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Plasmid Transformation of fshPCD

  • Plasmid name: fshPCD in V0120 vector
    • BioBrick name: BBa_J176002 BBa_J176002
    • Vector name: V0120 (BBa_J176127)
    • Ampicillin resistance on V0120
    • Length of vector: 4968 bp
  • Sample 1: Plasmid DNA - fshPCD (BBa_J176002)
  • Sample 2: negative control - water (Vol 1, p. 166)
  • Sample 1 had moderate growth from ~6:30 pm to ~10:30 am--transformation was successful.
  • Sample 2 had no growth save for a couple of colonies. Overall the transformation was successful.



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