Plasmid Extraction and Validation of fshPCD
- Name: fshPCD / BBa_J176002
- Date of culture inoculation: 9/26/13
- Results for Cultures 1 and 2: Mixture is cloudy so growth in both. Success.
- Results for Streaks 1 and 2: Growth in both areas along zig-zag line. Bottom left of agar plate dried out. Success.
- Sample 1: fshPCD/V0120 colony 1; BBa_J176002; part = 186 bp; vector = 4986 bp
- Sample 2: fshPCD/V0120 colony 2; same
- Sample 3: fshPCD/V0120 (control DNA); BBa_J176002; part = 186 bp; vector = 4986 bp
DNA Concentration Data
| Plasmid |
OD260 |
OD260/280 |
ng/μL
|
| 1. Plasmid 1 |
0.186 |
1.859 |
185.784
|
| 2. Plasmid 2 |
0.138 |
1.827 |
138.008
|
Restriction Digest Table
- Checked plasmid minipreps with EcoRI/PstI digests
| Reagent
|
Volume
|
Expected:
1. Plasmid 1 = 186 bp, 4986
2. Plasmid 2 = 186, 4986
|
15 μL/lane; 1% agarose; Ladder
|
| DNA(plasmid) |
3.0 μL
|
| 10X buffer |
1.5
|
| EcoRI |
1.0
|
| PstI |
1.0
|
| dH2O |
8.5
|
| |
15 μL --> 37°C/ 15 min.
|
DNA Sequencing Samples
- Submitted to DNASU on: 'date'
- Order Number:
- Plasmid 1 - forward primer 'name'
- Plasmid 1 - reverse primer 'name'
- Plasmid 2 - forward primer 'name'
- Plasmid 2 - reverse primer 'name'
|
Project name
