Haynes Lab:Notebook/Jan/2013/10/16: Difference between revisions
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== | ==Growing Liquid Culture of mCh== | ||
* | * mCh / BBa_J176005 | ||
** Transformation done on [http://openwetware.org/wiki/Haynes_Lab:Notebook/Jan/2013/10/15 10/15/13] | |||
** Result for Plate 1: Good growth over much of the plate. Overall success. | |||
** Result for Plate 2: Growth over about half of the plate, indicating an error in procedure. | |||
Because there was growth on the negative control plate, indicating a fault in the transformation, the plates were disposed of and the transformation was redone. | |||
* Plasmid name: mCh in V0120 vector | |||
** BioBrick name: BBa_J176005 [http://parts.igem.org/Part:BBa_J176005 BBa_J176005] | |||
** Vector name: V0120 (BBa_J176127) | |||
** Ampicillin resistance on V0120 | |||
** Length of vector: 3200 bp | |||
* Sample 1: Plasmid DNA - mCh (BBa_J176005) | |||
* Sample 2: negative control - water (Vol 1, p. 166) | |||
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Revision as of 16:47, 16 October 2013
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Growing Liquid Culture of mCh
Because there was growth on the negative control plate, indicating a fault in the transformation, the plates were disposed of and the transformation was redone.
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