Haynes Lab:Notebook/Jan/2013/10/18: Difference between revisions

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(Autocreate 2013/10/18 Entry for Haynes_Lab:Notebook/Jan)
 
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==Entry title==
==Extraction and Verification of mCh==
* Insert content here...
* Name: mCh / BBa_J176005
** Date of culture inoculation: [http://openwetware.org/wiki/Haynes_Lab:Notebook/Jan/2013/09/26 10/17/13]
** Results for Cultures 1 and 2: Mixture is cloudy so growth in both. Success.
** Results for Streaks 1 and 2: Growth in both areas along zig-zag line, although more growth in colony 1 streak than colony 2 streak. Overall success.


* Sample 1: mCh/V0120 colony 1; BBa_J176005; part = 705 bp; vector = 3200 bp
* Sample 2: mCh/V0120 colony 2; same
* Sample 3: mCh/V0120 (control DNA); BBa_J176005; part = 705 bp; vector = 3200 bp
'''DNA Concentration Data'''
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{| {{table}}
|- bgcolor=#cfcfcf
| Plasmid || OD260 || OD260/280 || ng/μL
|-
| 1. Plasmid 1 || --- || --- || ---
|-
| 2. Plasmid 2 || --- || --- || ---
|}
'''Restriction Digest Table'''<br>
* Checked plasmid minipreps with EcoRI/PstI digests
{| {{table}} border="1" cellspacing="3"
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<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2
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|- valign="top"
| bgcolor=#cfcfcf | Reagent
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = insert size, vector size<br>2. Plasmid 2 = insert size, vector size<br>
| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
|-
| DNA(plasmid) || 3.0 μL
|-
| 10X buffer || 1.5
|-
| EcoRI || 1.0
|-
| PstI || 1.0
|-
| dH<sub>2</sub>O || 8.5
|-
| &nbsp; || 15 μL --> 37°C/ 15 min.
|}
'''DNA Sequencing Samples'''
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* Submitted to DNASU on: 'date'
* Order Number:
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. -->
# Plasmid 1 - forward primer 'name'
# Plasmid 1 - reverse primer 'name'
# Plasmid 2 - forward primer 'name'
# Plasmid 2 - reverse primer 'name'


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Revision as of 09:40, 18 October 2013

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Extraction and Verification of mCh

  • Name: mCh / BBa_J176005
    • Date of culture inoculation: 10/17/13
    • Results for Cultures 1 and 2: Mixture is cloudy so growth in both. Success.
    • Results for Streaks 1 and 2: Growth in both areas along zig-zag line, although more growth in colony 1 streak than colony 2 streak. Overall success.
  • Sample 1: mCh/V0120 colony 1; BBa_J176005; part = 705 bp; vector = 3200 bp
  • Sample 2: mCh/V0120 colony 2; same
  • Sample 3: mCh/V0120 (control DNA); BBa_J176005; part = 705 bp; vector = 3200 bp


DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 --- --- ---
2. Plasmid 2 --- --- ---


Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
 Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on: 'date'
  • Order Number:
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'


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