Haynes Lab:Notebook/Jan/2013/10/18

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(Extraction and Verification of mCh)
(Extraction and Verification of mCh)
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* Order Number: 8258
* Order Number: 8258
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# Plasmid 1 - forward primer 'name'
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# mCh - forward primer 'P0001' -- 705 matches, thus 100% matching across the sequence. DNA cloning successful.
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# Plasmid 1 - reverse primer 'name'
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# mCh - reverse primer 'P0002' -- 705 matches, so 100% match. DNA cloning successful.
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# Plasmid 2 - forward primer 'name'
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# Plasmid 2 - reverse primer 'name'
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Revision as of 14:03, 9 November 2013

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Extraction and Verification of mCh

  • Name: mCh / BBa_J176005
    • Date of culture inoculation: 10/17/13
    • Results for Cultures 1 and 2: Mixture is cloudy so growth in both. Success.
    • Results for Streaks 1 and 2: Growth in both areas along zig-zag line, although more growth in colony 1 streak than colony 2 streak. Overall success.
  • Sample 1: mCh/V0120 colony 1; BBa_J176005; part = 705 bp; vector = 3200 bp
  • Sample 2: mCh/V0120 colony 2; same
  • Sample 3: mCh/V0120 (control DNA); BBa_J176005; part = 705 bp; vector = 3200 bp


DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 0.12 1.84 120
2. Plasmid 2 0.026 1.656 26


Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = 705 bp, 3200 bp
2. Plasmid 2 = 705 bp, 3200 bp
Image:GelImage.jpg
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on: '10/21/13'
  • Order Number: 8258
  1. mCh - forward primer 'P0001' -- 705 matches, thus 100% matching across the sequence. DNA cloning successful.
  2. mCh - reverse primer 'P0002' -- 705 matches, so 100% match. DNA cloning successful.



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