Extraction and Verification of mCh
- Name: mCh / BBa_J176005
- Date of culture inoculation: 10/17/13
- Results for Cultures 1 and 2: Mixture is cloudy so growth in both. Success.
- Results for Streaks 1 and 2: Growth in both areas along zig-zag line, although more growth in colony 1 streak than colony 2 streak. Overall success.
- Sample 1: mCh/V0120 colony 1; BBa_J176005; part = 705 bp; vector = 3200 bp
- Sample 2: mCh/V0120 colony 2; same
- Sample 3: mCh/V0120 (control DNA); BBa_J176005; part = 705 bp; vector = 3200 bp
DNA Concentration Data
| Plasmid |
OD260 |
OD260/280 |
ng/μL
|
| 1. Plasmid 1 |
0.12 |
1.84 |
120
|
| 2. Plasmid 2 |
0.026 |
1.656 |
26
|
Restriction Digest Table
- Checked plasmid minipreps with EcoRI/PstI digests
| Reagent
|
Volume
|
Expected:
1. Plasmid 1 = 705 bp, 3200 bp
2. Plasmid 2 = 705 bp, 3200 bp
|
15 μL/lane; 1% agarose; Ladder
|
| DNA(plasmid) |
3.0 μL
|
| 10X buffer |
1.5
|
| EcoRI |
1.0
|
| PstI |
1.0
|
| dH2O |
8.5
|
| |
15 μL --> 37°C/ 15 min.
|
DNA Sequencing Samples
- Submitted to DNASU on: '10/21/13'
- Order Number: 8258
- mCh - forward primer 'P0001' -- 705 matches, thus 100% matching across the sequence. DNA cloning successful.
- mCh - reverse primer 'P0002' -- 705 matches, so 100% match. DNA cloning successful.
|
Project name
